Syringe Filters | FAQ (2023)

Do you need a Chemical Compatibility Chart?

We have a Chemical Compatibility Chart that you can use for reference.

What is the filtration area of my syringe filter?

The syringe filter area, and other specifications, are readily available at Please go to the product page for your syringe filter and click the “Application/Specification” tab near the middle of the page. You can read the filter area from this tab; make sure to scroll down as necessary. Please contact [emailprotected] if you need assistance.

What syringe filters should I use for my HPLC samples?

Because of their low inherent levels of extractables, nylon membrane syringe filters are commonly used to filter samples that are intended for HPLC analysis. Nylon membrane syringe filters may not be suitable for all samples and are ideal for near neutral pH aqueous solutions and compatible nonaqueous solvents. For samples composed of acids and bases, hydrophilic PTFE membrane syringe filters and PES membrane syringe filters may be considered. For samples composed of nonaqueous solvents that are not compatible with nylon, PTFE membrane syringe filters and polypropylene membrane syringe filters may be considered. For aqueous samples containing proteins, or similar large organic molecules of interest, syringe filters containing membranes with low inherent protein binding characteristics, such as cellulose acetate (CA), should be considered to ensure maximum recovery.

How are the sterile syringe filters sterilized? How is sterility assured?

The standard Sterlitech sterile syringe filters are sterilized by controlled exposure to gamma irradiation. The amount of irradiation is carefully controlled to ensure a sufficient dose for sterility. The effectiveness of the treatment is monitored for each lot by using strategically placed spore strips and sterility is confirmed before a lot is released for sale.

Why would I select a syringe filter that has a glass fiber prefilter?

The glass fiber prefilter reduces the particle loading experienced by the membrane final filter and, consequently, extends the service life. For some applications with fluids containing high levels of suspended solids, a syringe filter with an incorporated glass fiber prefilter will usually have greater total throughput when comparted to one without a prefilter.

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Are syringe filters from different suppliers equivalent?

It would be expected that syringe filters containing absolute rated membrane media would be approximately equivalent regardless of supplier for filters that have the same size, same membrane material and same pore size rating. This is not the case for syringe filters containing nominally rated media such as glass fiber or nonwoven polypropylene. When considering alternative suppliers for nominally rated syringe filters, the user cannot typically rely on pore size rating alone. A more careful consideration of the media specifications, if available from the supplier, can help inform selection.

What is the maximum operating temperature for the Sterlitech syringe filters?

The maximum recommended operating temperature for the syringe filters when used with compatible fluids is 60°C. When operating at high temperatures, the housing strength is reduced and it may be necessary to limit the applied pressure. Appropriate safety precautions, such as the use of personal protective equipment (PPE), should be used when working with hot fluids.

What are the connections for the syringe filters? Can I purchase syringe filters with custom connections?

The standard Sterlitech syringe filters that are 30mm in size or smaller have female luer lock inlet connection and male luer slip outlet connection. For customers that require different connections, Sterlitech offers custom syringe filters with a variety of connection options for your convenience at Please contact [emailprotected] to inquire about availability and pricing.

Does the syringe filter size refer to the outer diameter of its housing?

No, the syringe filter size refers to the nominal diameter of the disk filter contained within. Necessarily, the outer diameter of the housing is somewhat larger than the labeled size. For example, the 25mm presterilized syringe filters offered by Sterlitech have a housing outer diameter of 33mm.

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Are the Sterlitech syringe filters suitable for pharmaceutical applications?

The syringe filters are primarily intended for laboratory use. The syringe filters maybe evaluated for pharmaceutical applications; however, it would be the responsibility of the user to comply with all pertinent regulations. Please contact [emailprotected] if you need assistance.

Can the syringe filters be autoclave sterlized?

Yes, some of the syringe filters offer by Sterlitech may be autoclave sterlized. The exceptions are syringe filters with mixed cellulose esters (MCE) membrane and syringe filters with acrylic housings.

Are syringes included with syringe filter orders?

No, syringes are not included with syringe filters orders. We may be able to offer syringes separately; please contact [emailprotected] for pricing and availability.

Does Sterlitech provide certificates of conformance (CoC) with syringe filter orders?

Yes, we can provide certificates of conformance (CoC) with syringe filter orders. Please make sure to the request the certificate when placing your order.

Can I still use my presterilized syringe filters after they have passed their expiration date?

Yes, the presterilized syringe filters may be used after passing their expiration date. However, we cannot guarantee that the filters are still sterile in the packaging. For applications that are sensitive to microbial contamination, the expired filters should be chemically sterilized, or gamma sterilized, prior to use. The expiration date only applies to filter sterility and is not relevant to filter performance. If stored sealed in their original packaging in climate controlled environments, it is expected that expired filters will have normal flow and retention characteristics.

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Can a syringe filter be used to prevent liquid from entering a vacuum pump?

Yes, for water and some aqueous solutions, a hydrophobic membrane syringe filter installed in the tubing between the vacuum pump and the receiving flask can prevent accidental overflows from reaching the vacuum pump. Sterlitech recommends PTFEVS4550BAR10 ( for this application. Please note that this method will not work for low surface tension fluids, such as alcohols and solvents.

What is the smallestpore size available for the Sterlitech syringe filters?

From our standard offering, the nylon membrane syringe filters ( have the smallest pore size rating available at 0.1µm.

Sterlitech can provide custom syringe filters ( with pore size ratings down to 0.05µm. Please contact [emailprotected] to inquire about availability and pricing.

What is the largest pore size available for the Sterlitech syringe filters?

From our standard offering, the largest pore size rating available is 5 microns. Bothcellulose acetate ( and nylon ( membrane syringe filters are available with 5 micron pore size rating.

Sterlitech can provide custom syringe filters ( with pore size ratings up to 250 microns. Please contact [emailprotected] to inquire about availability and pricing.

Do Sterlitech syringe filters contain surfactants?

Mostof the syringe filters offered by Sterlitech do not contain surfactants. The exceptions are syringe filters with cellulose acetate (CA) and mixed cellulose esters (MCE) membranes. CA and MCE membranesare treated with surfactants to ensure good wettability.

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Are Sterlitech Syringe Filters certified pyrogen free?

Q. Are Sterlitech Syringe Filters certified pyrogen free?

A. No, we do not perform pyrogen tests on our filters.

What is the maximum operating temperature for the 17mm and 30mm syringe filters?

Q. What is the maximum operating temperature for the 17mm and 30mm syringe filters?

A. The 17mm and 30mm syringe filters have a polypropylene housing, which allows them to withstand higher temperatures than an acrylic housing. The 17mm and 30mm's have a maximum operating temperature of 180°C. They are also able to be autoclaved.

What are the maximum filtration volumes for the 17mm and 30mm syringe filters?

Q. What are the maximum filtration volumes for the 17mm and 30mm syringe filters?

A. The 17mm and 30mm syringe filters offer greater filtration volume than smaller filters.

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  • 17 - 12ml
  • 17 with glass fiber prefilter - 20ml
  • 30 - 120ml
  • 30 with glass fiber prefilter - 180ml


Syringe Filters | FAQ? ›

0.45 um membranes are typically used for general filtration and particle removal while 0.2/0.22um membranes, or sterilizing-grade membranes, are most commonly used for solution sterilization (bacteria removal).

What is the difference between 0.45 and 0.22 syringe filter? ›

0.45 um membranes are typically used for general filtration and particle removal while 0.2/0.22um membranes, or sterilizing-grade membranes, are most commonly used for solution sterilization (bacteria removal).

What are syringe filters for? ›

Syringe filters are single-use, membrane-based devices used for the removal of particulate impurities from liquid and gas samples prior to analysis by methods such as HPLC, ion chromatography, gas chromatography, ICP, and dissolution testing.

What is a 0.2 micron syringe filter used for? ›

Syringe Filters by Pore Size

0.2/0.22 μm membranes, or sterilizing-grade membranes, are most commonly used for solution sterilization (bacteria removal).

What is the best syringe filter material? ›

Hydrophilic syringe filters are the most popular choice for filtering aqueous solutions. Nylon, polyethersulfone, and cellulose acetate syringe filters, to name a few, will efficiently and naturally filter water based solutions due to their hydrophilic properties.

What is a 0.45 micron filter used for? ›

The 0.45 µm pore size is used to recover bacteria and other microorganisms from many samples and environ- ments—almost to the exclusion of other pore sizes. Only rarely are other sizes used for growth and recovery and there is little information available on the effects of different pore sizes on microorganisms.

What is the difference between 0.2 micron and 0.22 micron filter? ›

What that means is, for the purpose of sterilization, 0.2 micron and 0.22 micron filters are indistinguishable. Their performance is the same, only the difference being the designation of their pore size rating.

Why use a 0.22 micron filter? ›

The 0.22-micron filter is one of the smallest used in patient care, and removes bacteria. There are not currently filters that remove viruses. Not all intravenous medications should be administered through a filter, and others may require filters of a specific size.

How many times can you use a syringe filter? ›

Never re-use a syringe filter, even if it looks perfectly clean. Whatman products filter out extremely fine particulate that may not be visible to the naked eye. Ensure the consistency of your results by discarding filters after a single use.

Can syringe filters be reused? ›

Syringe filters, which are about the size of a checkers game piece, are a key part of any lab setting but are not reusable and can make up a large portion of waste from a facility.

Can bacteria pass through 0.45 filter? ›

Bacteria growing on MF-Millipore filters (thickness, 150 µm) passed through the underlying membrane by their infiltration activity. Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, and Escherichia coli passed through a 0.45-µm pore size filter within 48–96 h.

Which is better .5 or 1 micron filter? ›

The smaller the micron number the better. Try imagining microns like a sieve. A 5 micron water filter will sieve out particles that you can see – but all the other smaller particles will pass through it into your drinking water. By contrast a 1 micron filter will remove particles not visible to the naked eye.

Will 0.45 Um filter remove bacteria? ›

General description. Filter type HA, 0.45 μm pore size, is designed to give complete retention and maximum recovery of total coliforms and fecal coliform bacteria.

How do I choose a syringe filter? ›

When choosing a syringe filter, three main factors dictate your syringe filter selection: sample volume, potential particulate size, and filter membrane compatibility.
  1. Diameter of the Syringe Filter and Sample Volume. ...
  2. Syringe Filter Pore Size. ...
  3. Syringe Filter Membrane Options. ...
  4. How Do I Use a Syringe Filter?
Dec 7, 2020

What is difference between 0.45 and 0.22 micron filter? ›

The 0.45 µm mixed esters of cellulose filter is the currently accepted membrane for this purpose and is typically used by Validation Services. The 0.22 µm filters, despite their ability to retain higher levels of bacteria, proved not to have an advantage over 0.45 µm membranes in terms of bacterial recovery.

What filter is most efficient? ›

HEPA, or High-Efficiency Particulate Air, filters have even higher MERV ratings, ranging from 17 to 20. These filters are extremely efficient and are used in cases where air quality is critical. They can remove almost all of the particulates in your facility's indoor air.

What is a 0.1 micron filter used for? ›

It can filter all types of food liquids, such as oil, wine or syrups. The 0.1 micron Polyray Cartridge is designed for use in a wide range of general process applications. It is made of high-impurity fiber-filled polypropylene. It is resistant to a large number of chemicals.

Do you want a higher or lower micron filter? ›

The smaller the micron rating, the faster the filter fills up. To prevent clogging, it is sometimes suggested to use more than one filter when there are a lot of particles, dirt, and debris to be filtered. A 5 micron rating works well in many industries, including the food and beverage industry.

Why do we use 0.3 micron filters? ›

This type of air filter can theoretically remove at least 99.97% of dust, pollen, mold, bacteria, and any airborne particles with a size of 0.3 microns (µm). The diameter specification of 0.3 microns corresponds to the worst case; the most penetrating particle size (MPPS).

How do I choose a micron filter? ›

The smaller the micron rating, the finer the particulate removed. Which size micron filter you need depends on the size of the smallest particle that needs filtering from your water. A 5-micron filter, for example, removes particles as small as 5 microns. Anything smaller passes through the pores.

Is 0.2 micron filter considered sterile? ›

Such 0.2 μm filtrations are frequently referred to as 'sterile filtration', reflecting the general belief that all living organisms are excluded from the filtrate. However, it has been demonstrated that a few bacterial species can pass through 0.2-μm filters.

Which is finer 5 micron or 50 micron? ›

The micron rating refers to the distance between pieces of filter media. This determines the size of particles that the filter will allow to pass through. A 50 micron rated filter will allow larger particles to pass through than a 5 micron rated filter.

Can viruses pass through 0.22 micron filter? ›

Some cell-associated viruses may pass through 0.22-μm or 0.45-μm filters and could be lost during the viral DNA extraction process if they are within ultrasmall bacteria that are common in aquatic environments (24).

Which is a finer filter 10 micron or 30 micron? ›

Filters with a larger micron rating allow larger particles through the system. Usually, a 10-micron filter is used for unleaded gasoline, and a 30-micron filter is used for diesel fuel. A 30-micron filter used in an unleaded dispenser unit can allow unwanted particles to pass through to the customer's vehicle.

Does a 0.22 micron filter remove bacteria? ›

Critical Process Filtration Sterilizing Filter Options

Critical Process Filtration offers the following choices for sterilizing filtration. All are validated for bacteria removal (0.22 and 0.1 microns), mycoplasma reduction (0.1 microns), and mycoplasma removal (0.03 microns).

Can I use the same syringe twice on myself? ›

It is not safe to change the needle and reuse the syringe – this practice can transmit disease.

Can I use the same syringe twice on same person? ›

Healthcare providers (doctors, nurses, and anyone providing injections) should never reuse a needle or syringe either from one patient to another or to withdraw medication from a vial. Both needle and syringe must be discarded once they have been used.

Do syringe filters need to be sterile? ›

Sterile syringe filters are used for filtering samples that need to be free from microbial contamination (such medical applications). Non-sterile syringe filters are typically used for filtering non-sterile solutions, such as for sample preparation in analytical chemistry.

Can you clean syringe filters? ›

Cleaning Syringe Filters

For cleaning the syringe filter, first the distilled water is pumped repeatedly with a needle tube, then it is subjected to ultrasonic for around 20 minutes, then it is soaked in distilled water for more than 5 hours, and finally it is withdrawn and dried at approximately 40 degrees.

What is the shelf life of syringe filters? ›

Nalgene sterile syringe filters have a three-year shelf life, with lot number and expiration date printed on individual syringe filter packages for easy traceability.

Will a 1 micron filter remove bacteria? ›

Look for a filter that has a pore size of 1 micron or less. This will remove microbes 1 micron or greater in diameter (Cryptosporidium, Giardia).

Which filter kills bacteria? ›

Ultrafiltration removes bacteria and viruses.

Ultrafiltration membrane technology is the latest and greatest in water purification for removing bacteria and viruses. It purifies and disinfects water to produce great-tasting water that is safe to use and drink.

Which filter is most efficient in removing bacteria? ›

Reverse osmosis filters and distillers are the most sophisticated systems. They are capable of removing bacteria and most residual chemicals, including minerals from a water source.

How many microns is a coffee filter? ›

Typically, coffee filters are made up of filaments approximately 20 micrometres wide, which allow particles through that are less than approximately 10 to 15 micrometres.

How many microns is a human hair? ›

A human hair is approximately 70 microns, give or take 20 microns depending on the thickness of a given individual's hair.

How often should you change a 5 micron filter? ›

The majority of systems use the following: 2 – 5-micron sediment filters, stage 1 (change every 6 months) 4 – 5- micron carbon filters, stage 2 and 3 (change every 6 months)

What size filter is bacteria proof? ›

bacterial filter A filter fine enough to prevent the passage of bacteria (0.5–5 μm in diameter), which permits removal of bacteria from solutions. Viruses are considerably smaller, and will pass through a bacterial filter.

Will a .1 micron filter remove viruses? ›

Key Takeaway: A water filter's micron rating is important for determining the quality of drinking water. 0.5-micron filters can remove most bacteria and cysts, 1-micron filters can remove bacteria, parasites and some viruses like HAV, while 5-micron filters are ideal for sediment removal without sacrificing flow rate.

What micron size is a sterile filter? ›

Sterile membrane filters. Filtering through a 0.2 µm pore size is considered necessary to produce a sterile filtrate.

How do I choose the right filter? ›

Things to consider when choosing a filter:
  1. Filter size and model.
  2. Original vs. aftermarket.
  3. Home air quality.
  4. Specific breathing needs.
  5. Type of filter.
  6. Filter performance.
  7. Filter cost and replacement.

Can you inject with a filter needle? ›

Filter Needle: A filter needle has a 5 micron filter at the base of a syringe needle. The filter creates a one-way flow when withdrawing or injecting fluid into or from the syringe. The filter needle can be used either to withdraw or to inject but never for both; it should only be pulled or pushed in one direction.

How do I know what order filter to use? ›

The order, n of a filter is the number of reactive elements (if all are contributing.) Using the linear slope (on log-log grid) away from f breakpoint it will be 6dB/octave per order of n. An n= 4th order is 24dB/octave slope as in both of 1st examples .

What are the three most commonly used filters? ›

Any other substances present within the water also affect the type of filter required for the system. Three common filter types for particle filtration are bag, cartridge, and self-cleaning filters.

What is the most commonly used filter? ›

Today, UV is the most widely commonly used filter, but now its role is different. It serves mainly to protect a lens from scratching and damage. Every photographer who cares about their equipment should use a UV filter. The UV filter in detail.

What is a better filter 10 micron or 100 micron? ›

Since a 10 micron filter is too restrictive on the inlet side, a less restrictive filter is preferred. For inline pumps where it is located outside the tank, a 100 micron pre-filter is recommended.

What is a 0.22 micron filter used for? ›

The 0.22-micron filter is one of the smallest used in patient care, and removes bacteria. There are not currently filters that remove viruses. Not all intravenous medications should be administered through a filter, and others may require filters of a specific size.

What is the purpose of 0.22 UM filter? ›

0.22 μm membrane filter for particulate and bacteria-free water at the point of dispense for the Milli-Q® IQ, IX and EQ 7 series water purification systems.

What can pass through a 0.22 micron filter? ›

Scanning electron microscopy showed bacteria passing-through the 0.22- micro m filter. Millipore membrane filters having well-defined reticulate structures will be useful in the study of infiltration activity of microbes.

What is the main purpose of filtering your filtrate with filters with pore size of 0.22 μm? ›

0.22 µm membrane pores will exclude most microbial contaminants (but not mycoplasma)

Does 0.45 Um filter remove bacteria? ›

0.45um is generally used to reduce microbial load, it can filter out most microorganisms and bacteria, and it can be used for filtration of conventional samples and mobile phases.

What size micron filter should I use? ›

What is better 1-micron or 5-micron? Generally speaking, a 5 micron filter is better for removing larger particles from your water such as sediment and rust, while a 1 micron filter is better for capturing smaller particles like bacteria and cysts.

What is the difference between a 1.2 micron filter and a 0.22 micron filter? ›

There are two main IV filter pore sizes; the 0.2 micron filter is used for aqueous solutions, and the 1.2 micron filter is recommended for larger molecule solutions such as lipids. The 0.2 micron filter has also been reported to remove air, microorganisms and particulate matter.


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